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| LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫. |
| We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in... |
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Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946 |
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| TAKAHASHI, Hiroto; HANEDA, Shingo; KAYANO, Mitsunori; MATSUI, Motozumi; 羽田, 真悟; 茅野, 光範; 松井, 基純. |
| Because the establishment of pregnancy begins at the uterine horn ipsilateral to the corpus luteum (ipsi-horn) in cattle, levels of progesterone (P4) and receptor expression in the endometrial tissue, which regulate the intrauterine environment for embryo development, may differ between the ipsi-horn and the uterine horn contralateral to corpus luteum (contra-horn). The aim of the present study was to determine the endometrial tissue P4 concentrations and nuclear progesterone receptor (PGR), progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 mRNA expressions in the cranial and middle parts of the uterine horns during the luteal phase. The results showed higher endometrial tissue P4 concentrations in the cranial part of the ipsi-horn than in... |
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Palavras-chave: Bovine; Progesterone; Receptor; Uterus. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4364 |
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| TSUZUKI, Nao; SEO, Jong-Pil; YAMADA, Kazutaka; HANEDA, Shingo; TABATA, Yasuhiko; SASAKI, Naoki; 羽田, 真悟; 佐々木, 直樹. |
| Platelet-rich plasma (PRP) was administered to cattle with hoof defects (10-mm drill hole) to promote sole defect healing. Two types of gels were prepared. One was a mixed gel [compound of gelatin microspheres (GM) incorporated with PRP and alginate] and was used for the test group, while the other was an alginate gel used for the control group. Each hole was filled with one of these gels. The drill hole depth, pressure pain, and hardness of the regenerated tissue were measured for 3 weeks after treatment. These measurements improved significantly in the test group than in the control group. The results suggest that a compound of GM incorporated with PRP and alginate had a hoof-regenerating effect in cattle. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3889 |
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| MIKI, Wataru; ONIYAMA, Hiroyuki; TAKEDA, Naomasa; KIMURA, Yuki; HANEDA, Shingo; MATSUI, Motozumi; TAYA, Kazuyoshi; NAMBO, Yasuo. |
| We observed structural changes in the follicles and uterus of heavy draft mares during estrus and examined the effect of a single injection of the gonadotropin-releasing hormone analog buserelin on ovulation and endocrine profiles. Twenty-two heavy draft mares were divided into a buserelin-treated group (n=8) and a control group (n=14). Mares were given an intramuscular injection of 40 µg buserelin when they presented signs of estrus to a teaser stallion, had ≥45 mm diameter follicles, and presented decreased uterine edema compared with the previous examination. The follicles and uterus were monitored using transrectal ultrasound imaging and measurement of blood levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone, and... |
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Palavras-chave: GnRH analog buserelin; Heavy draft mares; Ovulation; Single injection; Uterine edema. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4397 |
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| MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純. |
| The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =... |
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Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147 |
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| LEE, Hsu-Hsun; KIDA, Katsuya; MIURA, Ryotaro; INOKUMA, Hisashi; MIYAMOTO, Akio; KAWASHIMA, Chiho; HANEDA, Shingo; MIYAKE, Yoh-Ichi; MATSUI, Motozumi; 猪熊, 壽; 宮本, 明夫; 川島, 千帆; 羽田, 真悟; 松井, 基純. |
| In peripartum dairy cows, insulin resistance (IR) increases to adjust the direction of energy to lactation after calving. To investigate the effect of prepartum IR on postpartum reproductive performance, the insulin tolerance test (ITT) was applied to 15 cows at 3 weeks (Pre21) and 10 days (Pre10) before the predicted calving date. Blood glucose area under the curve (AUCglu) within 120 min after administration of 0.05 IU/kg-BW insulin was calculated. The occurrence of first ovulation, days to first artificial insemination (AI) and first AI conception rate were recorded. Nutritional status postpartum was evaluated by blood chemical analysis. Based on AUCglu changes from Pre21 to Pre10, cows were classified into either the AUC-up group (AUCglu increase, n=5)... |
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Palavras-chave: Dairy cows; Glucose metabolism; Insulin tolerance test; Reproductive performance; Transition period. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4129 |
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| 岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka. |
| 牛臨床におけるComputed Radiography (CR) の有用性を確立するため，管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性，鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが，粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく， IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results，it was concluded that accidental variations in the tube-cassette... |
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Palavras-chave: 牛; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939 |
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| 内山, 裕貴; 都築, 直; 徐, 鍾筆; 山田, 一孝; 羽田, 真悟; 眞鍋, 弘行; 田畑, 泰彦; 佐々木, 直樹; HANEDA, Shingo; SASAKI, Naoki. |
| 自家血液から作製された多血小板血漿（Platelet rich plasma，以下PRP）は，アルギン酸ゲル剤として患部に塗布することで蹄底潰瘍への良好な治癒効果が認められた．しかし，効果を持続させるという点で不十分な方法であった．そこで本研究では，徐放剤としてゼラチンマイクロスフィア（以下 GM）を用いた新しい塗布剤を作製し，治療効果を検討した．PRP含浸GM混合アルギン酸ゲル処置群（PRP群，n＝10）とアルギン酸ゲルのみ処置群 （対照群，n＝9）を比較した．蹄底潰瘍面積の欠損割合は，PRP群で処置後1，2及び3週目で約45％，17％及び5％と推移し，対照群と比較して有意な角質再生効果がみられた．また，PRP群では処置後2週目に圧痛が消失したが，対照群では痛みは継続していた．以上のことから，PRP含浸GM混合アルギン酸ゲルは乳牛の蹄底潰瘍に有効であることが明らかとなった． |
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Palavras-chave: ゼラチンマイクロスフィア; 多血小板血漿 蹄底潰瘍. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3886 |
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| 佐々木, 直樹; 山田, 一孝; 石井, 三都夫; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 古岡, 秀文; 古林, 与志安; 猪熊, 壽; SASAKI, Naoki; YAMADA, Kazutaka; ISHII, Mitsuo; Matsui, Motozumi; HANEDA, Shingo; Matsumoto, Kotaro; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4378 |
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